Journal: International journal of molecular sciences

Article Title: Topical Molecular Iodine: An Optimal Biocide Constrained by Inadequate Formulations.

doi: 10.3390/ijms26104853

Figure Lengend Snippet: Figure 1. Human skin cell viability of carrier (glycerin) and positive controls. Skin explants were maintained in tissue culture and 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT) reagent was used to evaluate mitochondrial activity. The reported value is the average (n = 3) cell viability normalized to a phosphate buffer (PBS) at each time point. Test agents included the following established human skin irritants (10% Tween 20, 1% Triton X-100, and 5% sodium dodecyl sulfate).

Article Snippet: Explants were then incubated at 37 ◦C for 3, 7, or 24 h. Cell viability was evaluated (n = 3 for each timepoint/treatment) using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT) reagent from R&D Systems (Minneapolis, MN, USA).

Techniques: Activity Assay

Journal: International journal of molecular sciences

Article Title: Topical Molecular Iodine: An Optimal Biocide Constrained by Inadequate Formulations.

doi: 10.3390/ijms26104853

Figure Lengend Snippet: Figure 2. Human skin cell viability versus I2 concentration. I2 (500 to 15,000 ppm) in glycerin. Skin explants were maintained in tissue culture and 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT) reagent was used to evaluate mitochondrial activity. The reported value is the average (n = 3) cell viability normalized to a phosphate buffer (PBS) at each time point. The dashed line (---) at each timepoint displays the normalized cell viability value that corresponds to 50% of the PBS control.

Article Snippet: Explants were then incubated at 37 ◦C for 3, 7, or 24 h. Cell viability was evaluated (n = 3 for each timepoint/treatment) using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT) reagent from R&D Systems (Minneapolis, MN, USA).

Techniques: Concentration Assay, Activity Assay, Control